Characterization of Single Stranded Binding Protein’s Nucleic Acid Binding Requirements
Protein-nucleic acid interactions are critical during all phases of the life of a cell. Among the proteins that bind nucleic acids are those that preferentially bind to single stranded DNA. One such protein, the single stranded DNA binding protein (SSB) from E. coli is involved in cellular reproduction and maintenance of genetic information (Krauss et al. 1981). In this application note we demonstrate the capability of the BiOptix 404Pi™ to analyzing protein-oligonucleotide interactions in real time and without the need of prior molecular labeling (Meyer and Laine, 1990; Kozlov and Lohman, 2002).